Fast, reliable detection of pathogenic Escherichia coli helps you make product release decisions with confidence.
| BAX® System Real-Time PCR Assay for E. coli O157:H7 |
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Developed in collaboration with the USDA Agricultural Research Service and validated on real-world sample sizes, this extremely reliable and highly accurate test uses probe-based chemistry for advanced real-time analysis to detect all known E. coli O157:H7—even atypical isolates—in less than an hour processing time. |
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| Specifications |
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| Target |
E. coli O157:H7 |
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| Matrices |
Raw ground beef, beef trim, spinach, lettuce |
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| Enrichment medium |
BAX® System MP media |
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| Enrichment time |
9 hours for 65g ground beef; 10 hours for 375g beef trim; 8 hours for 25g spinach & lettuc |
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| PCR time |
55 minutes |
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| BAX instruments |
Q7 |
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| Detection level |
10^4 cfu/mL |
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| Certifications |
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Performance Tested Method #031002 validated on raw ground beef, beef trim, spinach and lettuce. This test kit's performance was reviewed by AOAC Research Institute and was found to perform to the manufacturer's specification.
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QUA-18/07-07/10 certified according to AFNOR validation rules for raw beef and vegetables.
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Approvals
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| Health Canada |
MFLP-76 validated on raw ground beef and beef trim.
The USDA-FSIS has approved Health Canada's MFLP-76 as equivalent to the method used by FSIS to detect E. coli O157:H7 in raw ground beef and beef trim.
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Ordering info
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| D14203648 |
BAX System Real-Time PCR assay for E. coli O157:H7
(96 tests per kit) |
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| BAX® System Real-Time PCR STEC Suite |
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Aligned with the USDA FSIS approach, the STEC screening assay detects virulence genes stx and eae to clear negative samples quickly and cost-effectively. Using the same lysate, panel assays determine if positive screening samples contain one of the top six serogroups of non-O157 Shiga toxin-producing E. coli.
Learn more about this quick and convenient method |
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| Specifications |
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STEC Screening: stx and eae |
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STEC Panel 1: E. coli O26, O111, O121 |
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STEC Panel 2: E. coli O45, O103, O145
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| Matrices |
Ground beef, beef trim, produce
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| Enrichment medium |
TSB (TSB+n for high volume ground beef) or MP media |
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| Enrichment time |
Minimum 9-12 hours depending on food type and volume |
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| PCR time |
55 minutes |
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| BAX instruments |
Q7 |
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| Detection level |
10^4 cfu/mL |
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Ordering info
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| D14642964 |
BAX® System Real-Time PCR Assay: STEC Screening for stx and eae (96 tests) |
| D14642970 |
BAX® System Real-Time PCR Assay: STEC Panel 1 for E. coli O26, O111, O121 (48 tests) |
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| D14642987 |
BAX® System Real-Time PCR Assay: STEC Panel 2 for E. coli O45, O103, O145 (48 tests) |
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| BAX® System PCR Assay for E coli O157:H7 MP |
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This multiplex (MP) assay includes several targets on the E. coli O157:H7 genome for greater assurance that new, unusual or modified varieties of this complex organism are detected. |
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| Specifications |
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| Target |
E. coli O157:H7 |
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| Matrices |
Raw ground beef, beef trim, spinach and lettuce |
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| Enrichment medium |
BAX® System MP media |
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| Enrichment time |
8-24 hours |
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| PCR time |
3.5 hours |
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| BAX instruments |
Q7 or Classic |
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| Detection level |
10^4 cfu/mL |
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| Adoptions |
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| USDA FSIS |
MLG 5A.02 - raw ground beef and raw beef trim |
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| Certifications |
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Performance Tested Method #050501 validated on raw ground beef, beef trim, spinach and lettuce. This test kit's performance was reviewed by AOAC Research Institute and was found to perform to the manufacturer's specification.
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QUA-18/04 03-08 certified according to AFNOR validation rules for raw beef in BAX® System MP media 8-24 hr; raw milk, fruits, vegetables and ready-to-eat meals, raw pork meat, raw horse meat and raw chicken meat in mTSB+novobiocin 18-24 hr - applies to Q7 instruments. |
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Approvals
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| Health Canada |
Supplement 2 to MFLP#30 applies to dairy, meat, fruit and vegetables (after 18-24 hours in modified tryptic soy broth with novobiocin, mTSB-n) and to dry foods, environmentals and animal feed (after 1 hour in BPW, then 18-24 hours in mTSB-n). Also applies to ground beef and beef trim after 8-24 hours in BAX® System MP media. |
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Ordering info
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| D12404903 |
BAX® System PCR Assay for Screening E. coli O157:H7 MP
(96 tests per kit) |
| D12404925 |
BAX® System MP Media
(2.5 kg per tub) |
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