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BAX® System PCR Assay

Enterobacter sakazakii

When Enterobacter sakazakii is present in a food sample, the genetic-based BAX® system uses the power of the polymerase chain reaction (PCR) to rapidly create (amplify) millions of copies of a unique, E. sakazakii -specific DNA fragment for reliable detection.

BAX® system assays simplify the method by combining all the PCR reagents you need into a single tablet, conveniently packaged inside the PCR tubes contained in each kit. Tableted reagents provide for minimal hands-on time, in addition to accuracy, long shelf life and consistency.

After standard enrichment, samples are lysed to break open the cell walls and release DNA. This lysate is then used to hydrate the PCR tablets. Processing in the automated unit takes less than 4 hours for a full rack of 96 tests. Electronic results appear as positive/negative icons on screen, and the data can be easily stored, shared or printed.

Applications

The BAX® system detects Enterobacter sakazakiii in selected foods, including powdered infant formula, dry dairy and soy ingredients and food production environmental samples. 

Validation studies have shown that the BAX® system, with 98% sensitivity/specificity rates,  performs as well as traditional culture methods, but with next-day results.

Ordering Info

P/N D11801836 (previously P/N 17720657)   BAX® System PCR Assay for Screening Enterobacter sakazakii (96 tests per kit)