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BAX® System PCR Assay

Genus Listeria

When Listeria is present in food or environmental samples, the genetic-based BAX® system uses the power of the polymerase chain reaction (PCR) to rapidly create (amplify) millions of copies of a unique, Listeria -specific DNA fragment for reliable detection.

BAX® system assays simplify the method by combining all the PCR reagents you need into a single tablet, conveniently packaged inside the PCR tubes contained in each kit. Tableted reagents provide for minimal hands-on time, in addition to accuracy, long shelf life and consistency.

After standard enrichment, samples are lysed to break open the cell walls and release DNA. This lysate is then used to hydrate the PCR tablets. Processing in the automated unit takes less than 4 hours for a full rack of 96 tests. Electronic results appear as positive/negative icons on screen, and the data can be easily stored, shared or printed.

Applications

The BAX® system detects Listeria in foods, including dairy, meat, fish and vegetables, and on environmental surfaces, including ceramic, plastic, wood, iron, concrete and more.

For environmental samples, proprietary BAX® system Listeria media combines a single enrichment step with shorter incubation times (24 hours for swabs and 28 hours for sponges) for quicker time to result.

Validation studies have shown that the single-enrichment BAX® system performs as well at 24-28 hours as the dual-enrichment USDA-FSIS culture method at 48 hours.

For food and environmental samples, standard media provides a simple 2-step enrichment for detecting Listeria .

With specificity rate of >99%, the BAX® sytem for detecting Listeria has been certified by AOAC Research Institute as a Performance Tested method. 

Approvals

  • Health Canada MFLP-15e - wide variety of environmental surfaces
  • Brazil MAPA Directive MLG 8A.01
  • AOAC-RI Performance Tested Method 030502 - tested on plastic, ceramic tile, rubber, painted and unpainted wood, sealed concrete, cast iron and air filter material. The approval has also been extended to include dairy, meat, fish, vegetables and environmental samples with a two-step enrichment. This assay has been validated for use with both the original and BAX® System Q7 instruments.

  Samples of this test kit model were independently evaluated by the AOAC Research Institute and were found to perform tothe producer's specifications as stated in the test kit's descriptive insert. The producer certifies this kit conforms in all respects to the specifications originally evaluated by the AOAC Research Institute as detailed in the Performance Tested Certificate number 030502

Ordering Info

P/N D11000147 (previously P/N 17710610)   BAX® System PCR Assay for Screening Genus Listeria (96 tests per kit)

P/N D12232794 (previously P/N 17710672)   BAX® System Media for Listeria (2.5 kg  per tub - makes 41 liters)