| Introducing a new BAX® system assay that uses reverse-transcriptase PCR to quickly and accurately detect Listeria in just 8 hours. With results that fast, it’s easy to test environmental samples throughout the production process— from HVAC systems to drains, conveyers and equipment. By the end of the day, you can know if your product is ready to ship, or if intervention is needed—limiting the risk of product contamination to just one shift. |
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View an animation of the science behind the new BAX® System 8-hour assay for detecting Listeria. |
Introducing a new BAX® system assay that uses reverse-transcriptase PCR to quickly and accurately detect Listeria in just 8 hours. With results that fast, it’s easy to test environmental samples throughout the production process— from HVAC systems to drains, conveyers and equipment. By the end of the day, you can know if your product is ready to ship, or if intervention is needed—limiting the risk of product contamination to just one shift.
Reverse-transcriptase PCR has been used successfully in the clinical and diagnostic industries, but this new PCR assay for the BAX® System is the first commercial application of reverse-transcriptase technology to bacterial testing in food.
Much like DNA, bacteria possess specific RNA sequences that are unique to a targeted organism. Although each bacterial cell has one or perhaps a few DNA molecules, it can typically contain thousands of RNA molecules.
Reverse-transcriptase PCR is used to initially synthesize complimentary DNA from RNA strands. The process then segues into standard DNA amplification and detection. The huge numbers of RNA fragments present at the start of the PCR reaction lead to dramatically improved sensitivity versus other detection methods.
The BAX® system detects all species of Listeria, even at very low concentrations (<10 cfu/mL for all species except L. grayi at <50 cfu/mL).
With reverse-transcriptase PCR, samples do not require the usual 24-48 hour enrichment in nutrient brotoh. Instead, Listeria cells are resuscitated by heating in the collection buffer solution for four hours, providing a jump-start to the process.
Validation studies using both the classic and Q7 models demonstrated BAX® system performance that was significantly superior to the reference USDA FSIS culture method in detecting Listeria from stainless steel surfaces. In a panel of 58 strains across the 7 species of Listeria and 52 strains of non-Listeria, the assay demonstrated 100% inclusivity/exclusivity.
AOAC-RI Performance Tested Method 030801 - tested on several types of stainless steel. This assay has been validated for use with both the original and BAX® Q7 systems.
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This test kit's performance was reviewed by the AOAC Research Institute and was found to perform to the manufacturer's specifications. |
D13602912 BAX® System Reverse-Transcriptase PCR Assay and Collection Kit for Listeria species (96 tests)
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