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BAX® System PCR assays for Listeria

The BAX® System offers three assays for detecting Listeria spp to accommodate your lab's needs. 

Also see BAX® System PCR assays for detecting L. monocytogenes 


BAX® System Real-Time PCR Assay for Genus Listeria
     
  With a simplified sample preparation procedure and rapid real-time processing, this BAX® System assay provides fast and accurate molecular testing for Listeria species in food and environmental samples.
     
Specifications  
     
 Target Listeria species (including L. monocytogenes, L. ivanovii, L. seeligeriL. welshimeriL. grayii, L. marthii and L. innocua)  
 Matrices  Dairy, meat, fish, vegetables, environmentals  
 Enrichment medium  24 LEB Complete or standard media (BLEB or UVM, followed by MOPS-BLEB)  
 Enrichment time

24-28 hours with 24 LEB Complete

44-72 hours with standard media

 
 PCR time  about 70 minutes  
 BAX instruments  Q7  
 Detection level  10^4 cfu/mL  
     

Certifications

 

 

 

Certified as Performance Tested MethodSM #081401 by the AOAC Research Institute as an effective method for the detection of Listeria spp. in foods and environmental surfaces. Validated using frankfurters, cooked shrimp, lettuce, queso fresco, stainless steel, plastic and concrete.  
   

Ordering info

 

 D15131113 BAX® System real-time PCR assay for Genus Listeria (96 tests per kit)
 D14654989 24 LEB Complete (2.5 kg) - Oxoid CM1154
 
 

 
 

 

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BAX® System PCR Assay for Genus Listeria 24E
     
  This assay detects even low concentrations of Listeria in food and environmental samples after overnight enrichment. Performance is comparable to culture but with faster time to result.
     
Specifications  
     
 Target  Listeria species (except L. grayi )  
 Matrices  Dairy, meat, fish, vegetables, environmentals  
 Enrichment medium  24 LEB  
 Enrichment time  24-28 hours  
 PCR time  3.5 hours  
 BAX instruments  Q7 and classic  
 Detection level  10^4 cfu/mL  
     
Certifications  
   
  Performance Tested Method #050903  validated on bagged spinach, processed cheese, frankfurters, cooked shrimp and stainless steel. This test kit's performance was reviewed by AOAC Research Institute and was found to perform to the manufacturer's specification. This certification has been extended for use with blended 24 LEB Complete media. 
 

QUA-18/06-07/08 certified according to AFNOR validation rules for all human food products* and environmentals.
*Smoked fish requires use of buffer supplement.

     

Ordering info

 

 D13608135 BAX® System PCR assay for Genus Listeria 24E (96 tests per kit)
 D14654989 24 LEB Complete (2.5 kg) - Oxoid CM1154
Note: Each 2.5kg tub makes 57.5 liters, sufficient for 640 environmental or 255 food samples.
 
 D14327522 24 LEB Buffer Supplement (24 bottles of 10 mL) - Oxoid B01204  


 
24 LEB is also available from Oxoid as separate components. 24 LEB Base (500 gm) - Oxoid CM1107
24 LEB Selective Supplement (10 vials) - Oxoid SR0243
 
 

Note: A full kit (96 tests) for food testing uses 2 bottles (1 kg) base and 5 packs (50 vials) supplement. A full kit for environmental testing uses 1 bottle (500 gm)base and 2 packs (20 vials) supplement.

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BAX® System PCR Assay for Genus Listeria
     
  This assay uses standard media to detect Listeria species in food and environmental samples after 48 hours enrichment.
     
Specifications  
 Target  Listeria species (except L. grayi)  
 Matrices  Food and environmental samples  
 Enrichment medium  Demi-Fraser and MOPS-BLEB  
 Enrichment time  ~48 hours  
 PCR time  3.5 hours  
 BAX instruments  Q7 and classic  
 Detection level 10^5 cfu/mL after enrichment  
     
Certifications  
  AOAC-RI Performance Tested Method 030502  - tested on frankfurters,smoked salmon, spinach, cheese and environmental sponges. This test kit's performance was reviewed by AOAC Research Institute and was found to perform to the manufacturer's specification.
     
Ordering info    
 D11000147 BAX® System PCR Assay for Genus Listeria (96 tests)
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