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BAX® System PCR assays for Listeria monocytogenes

The BAX® System offers two assays for detecting Listeria monocytogenes to accommodate your lab's needs. 

Also see BAX® System PCR assays for detecting Listeria spp.


BAX® System PCR Assay for L. monocytogenes 24E
     
  This assay detects even low concentrations of Listeria monocytogenes in food and environmental samples after overnight enrichment. Performance is comparable to culture but with faster time to result.
 

Specifications

 

 Target  Listeria monocytogenes  
 Matrices  Dairy, meat, fish, vegetables, environmentals  
 Enrichment medium  24 LEB  
 Enrichment time  24-28 hours  
 PCR time  3.5 hours  
 BAX instruments  Q7 and classic  
 Detection level  10^4 cfu/mL  
     

Certifications

 

 

Performance Tested Method #080901   validated on bagged spinach, processed cheese, frankfurters, cooked shrimp and stainless steel. This test kit's performance was reviewed by AOAC Research Institute and was found to perform to the manufacturer's specification. This certification has been extended for use with blended 24 LEB Complete media.

   
 

QUA-18/05-07/08 certified according to AFNOR validation rules for all human food products* and environmentals.
*Smoked fish and raw charcuteries require use of buffer supplement.

 

Ordering info

 

 D13608125 BAX® System PCR assay for L. monocytogenes 24E (96 tests per kit)
 D14654989 24 LEB Complete (2.5 kg) - Oxoid CM1154
Note: Each 2.5kg tub makes 57.5 liters, sufficient for 640 environmental or 255 food samples. 
 
 D14327522 24 LEB Buffer Supplement (24 bottles of 10 mL) - Oxoid B01204  


 
24 LEB is also available from Oxoid as separate components. 24 LEB Base (500 gm) - Oxoid CM1107
24 LEB Selective Supplement (10 vials) - Oxoid SR0243
 
 

Note: A full kit (96 tests) for food testing uses 2 bottles (1 kg) base and 5 packs (50 vials) supplement. A full kit for environmental testing uses 1 bottle (500 gm)base and 2 packs (20 vials) supplement.

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BAX® System PCR Assay for L. monocytogenes
     
This assay uses standard media to detect Listeria monocytogenes in food and environmental samples after 48 hours enrichment.
     
Specifications  
 Target  Listeria monocytogenes  
 Matrices  Variety of food  
 Enrichment medium  Demi-Fraser and MOPS-BLEB  
 Enrichment time  ~48 hours  
 PCR time  3.5 hours  
 BAX instruments  Q7 and classic  
 Detection level 10^5 cfu/mL after enrichment  
 
Adoptions
USDA-FSIS MLG 8A.04 -applies to red meat, poultry, eggs and environmental samples
 
Approvals
AOAC International Official Method 2003.12 - applies to dairy products, fruits and vegetables (except radishes), seafoods, raw and processed meats, and poultry
Health Canada MFLP 28 - applies to a wide variety of food, including raw meats, processed meats, seafood, fresh produce/vegetables, cultured and non-cultured dairy, egg and egg products, and fruit juices
Brazil MAPA MLG 8A.01 - beef, poultry, eggs and environmental samples
 
Certifications
AOAC-RI Performance Tested Method 070202  - applies to a wide variety of food. Teste on raw meats,  processed meats, seafood, dairy cultured/non-cultured, egg and egg products, fresh produce/vegetables, and fruit juices. The assay has been validated for use with both the classic and BAX® System Q7 instruments. This test kit's performance was reviewed by AOAC Research Institute and was found to perform to the manufacturer's specification.
 
Ordering info
 D11000157 BAX® System PCR Assay for L. monocytogenes (96 tests)
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