When Salmonella is present in a food or environmental sample, the genetic-based BAX® system uses the power of the polymerase chain reaction (PCR) to rapidly create (amplify) millions of copies of a unique, Salmonella -specific DNA fragment for reliable detection.
BAX® system assays simplify the method by combining all the PCR reagents you need into a single tablet, conveniently packaged inside the PCR tubes contained in each kit. Tableted reagents provide for minimal hands-on time, in addition to accuracy, long shelf life and consistency.
After standard enrichment, samples are lysed to break open the cell walls and release DNA. This lysate is then used to hydrate the PCR tablets. Processing in the automated unit takes less than 4 hours for a full rack of 96 tests. Electronic results appear as positive/negative icons on screen, and the data can be easily stored, shared or printed.
Applications
The BAX® system detects Salmonella in a variety of foods, including meat, poultry, fish and seafood, fruit and vegetable products, dairy products, chocolate/bakery products, animal feeds and pasta.
Validation studies have shown that the BAX® system performs as well as traditional culture methods for detecting Salmonella in food, but with quicker time to result.
With sensitivity/specificity rates of 98%, the BAX® sytem for detecting Salmonella has been adopted by the USDA-FSIS and approved by government agencies and certifying bodies around the world.
Adoptions
USDA-FSIS MLG 4C.02 - raw meat, carcass sponges, whole bird rinses, ready-to-eat meat and poultry products, and pasteurized egg products
Approvals
- AOAC Official Method 2003.09 - collaborative study on raw beef, raw chicken, raw frozen fish, cheese, frankfurters and orange juice
- USDA-APHIS NPIP - for environmental samples, cloacal swabs, chick box papers, and meconium samples
- Health Canada MFLP-29 - meat, poultry, fish and seafood, fruits and vegetables, dairy, and miscellaneous food products - applies to original and Q7 instruments
- Brazil MAPA Directive 41 - food, water and environmental samples
- AFNOR Certificate QUA-18/3-11/02(French) (English)- all human and animal food products and environmentals - applies to original and Q7 instruments
- NordVal Certificate 030 - food, animal feed and environmentals - applies to original and Q7 instruments
- Danish Veterinary and Food Administration - accepted as an alternative method for all foods and environmental samples - applies to original and Q7 instruments
- AOAC-RI Performance Tested Method 100201 - tested on milk, black pepper, chilled ready meal, chipped ham, chocolate, cooked chicken, cooked fish, custard, dry pet food, elbow macaroni, frozen peas, hot dogs, nonfat dry milk, orange juice, peanut butter, pizza dough, seafood-prawns, alfalfa sprouts, ground beef, and liquid egg. This assay has been validated for use with both the original and BAX® System Q7 instruments.
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Samples of this test kit model were independently evaluated by the AOAC Research Institute and were found to perform tothe producer's specifications as stated in the test kit's descriptive insert. The producer certifies this kit conforms in all respects to the specifications originally evaluated by the AOAC Research Institute as detailed in the Performance Tested Certificate number 100201 |
Ordering Info
P/N D11000133 (previously P/N 17710608) BAX® System PCR Assay for Screening Salmonella (96 tests per kit)
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